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Read Online »Genomic alterations in androgen independent prostate cancer cell line DU145 and its sublines in cell cycle-dependent expression of PS-1, a gialpha protein-associated multi-spanning membrane protein. DU145 cells form a slowly growing androgen-independent cell line, which was established in this laboratory. We examined genome alterations in DU145 cells and its sublines which have lost the androgen sensitivity. The expression of PS-1, a protein that was identified as a gialpha protein-associated multi-spanning membrane protein, was induced in DU145 cells and several of its sublines upon growth in the absence of androgen. The expression of PS-1 in DU145 cells was attenuated by the treatment of actinomycin D or alpha-amanitin, suggesting that PS-1 may be dependent on active transcription and, in addition, RNA processing. The PS-1 gene was amplified by the use of poly(A) reverse transcription-polymerase chain reaction and RT-PCR in DU145 cells and several of its sublines. Northern blot analysis indicated that PS-1 mRNA was alternatively spliced to form at least two mRNA species. These results indicate that PS-1 is one of the genes whose expression is regulated in a cell cycle-dependent manner.Q: How to get latitude and longitude from a JsonObject I have the following JsonObject that I want to convert into a List so that I can iterate over the items and print out the latitude and longitude fields of each item. { "version" : 3, "notify_url" : "", "timestamp" : 1374995809, "event" : "m.geomessage", "event_type" : "new", "locations" : [ { "type" : "Point", "coordinates" : [ 38.71327, -77.01997] } ], "receivers




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